Figure 4.

MiR-487b-5p induces allergic responses of HNEpCs in vitro by targeting TRIM13. (A) The sequences of 3ʹUTR for TRIM13, which are complementary to miR-487b-5p seed sequence. WT represent wild-type and Mut represent mutant sequences. (B and C) qRT-PCR analysis shows that the expression of TRIM13 was increased in nasal mucosa of the AR patients (n=23) compared with nasal mucosal tissues of healthy controls (n=21). And statistical analysis indicated that negative correlation between TRIM13 and miR-487b-5p in nasal mucosal tissues. (D and E) The luciferase reporter assays results demonstrated that the luciferase activity of TRIM13-wild-type version significantly increased when miR-487b-5p knockdown in HNEpCs, while decreased when miR-487b-5p mimics in HNEpCs. However, the luciferase activity of Trim13- mutant version was unchanged. (F) qRT-PCR analysis result showed that the expression mRNA level of TRIM13 was significantly reduced in miR-487b-5p mimics group, but obviously enhanced in miR-487b-5p inhibitor group. (G) Western blot analysis result showed that the expression protein level of Trim13, p65 and p-p65 was significantly reduced in miR-487b-5p mimics group, but obviously enhanced in miR-487b-5p inhibitor group. Data were presented as mean ± SEM. Data are pooled from 3 independent experiments.

Abbreviations: HNEpCs, human nasal epithelial cell line; UTR, untranslated region; AR, allergic rhinitis; SEM, standard error of mean.