Figure 7.

Knockdown hsa_circ_100791 increase the expression of miR-487b-5p and reduced the nasal symptoms of AR in OVA-induced AR mice. (A) Schematic diagram of mouse model. (B–D) qRT-PCR analysis of the expression of hsa_circ_100791, miR-487b-5p and TRIM13 in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. (E and F) The frequencies of nose rubbing and sneezing were record in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. (G–I) qRT-PCR analysis of the expression of IL-4, IL-5 and IL-6 in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. (J and K) HE analysis of the number of eosinophils in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. (L and M) IHC analysis of the expression protein level of Trim13 in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. (N) IF analysis showed that the Trim13 colocalized in the cytoplasm and the expression protein level of Trim13 in the OVA group or the OVA+ si-circ100791 or the OVA+ circ-NC group. Data were presented as mean ± SEM. N=6 for each group.

Abbreviations: AR, allergic rhinitis; OVA, ovalbumin; NC, negative control; HE, hematoxylin-eosin; SEM, standard error of mean.