Fig. 5.

Stat3 or Stat6 mediated to the LPS or IL-4 induced changing of Sirpα. Macrophages from the adductor muscles of wild-type mice were treated with 30 ng/ml LPS or IL-4 at various times. Lentivirus was used to knock down Stat3 (siStat3) or Stat6 (siStat6) in the macrophage. A, B The level of Stat3, phosphorylated Stat3 (pStat3), Stat6, and phosphorylated Stat6 (pStat6) in the macrophages that received LPS for various time (A), and the quantification (B, n = 3). C, D The level of Stat3, pStat3, Stat6, pStat6 in the macrophages that received IL-4 for various times (C), and the quantification (D, n = 3). E and F The levels of miR17 ~ 92 cluster in the macrophages that received LPS (E) or IL-4 (F), were determined by RT-qPCR (n = 4). G Stat3 and Sirpα levels in the LPS pre-treated Stat3 knockdown macrophages were examined. GAPDH was used as a control (n = 3). H Stat6 and Sirpα levels in the IL-4 pre-treated Stat6 knockdown macrophages were examined. GAPDH was used as a control (n = 3). Data is analyzed using one-way ANOVA followed by Tukey multiple range test and expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. ns, non-significant