Table 6.
Environmental swab metagenomic samples, from which Enterobacterales were isolated, showing which type of sample was taken (sponge swab, stick swab or wastewater sample), number of bacterial species identified (including Gram negatives and Gram positives), and number of reads obtained from metagenomic sequencing
| Environmental sample number | Room | Swab location | Total species found | Swab type | Total reads |
|---|---|---|---|---|---|
| 15 | Sluice | Domestic Waste skip, lid | 163 | Sponge swab | 2,613,760 |
| 35 | Pantry (staff & patient) | Drinks Cooler, pre-Flush | 53 | Wastewater | 1,032,506 |
| 41 | Drug Prep Room | Sink drain, U bend exterior | 44 | Sponge swab | 790,760 |
| 60 | Workstation on wheels | Keyboard and Mouse | 34 | Sponge swab | 864,837 |
| 71 | Bay (Beds 1–4) | Bathroom HWB Drain | 74 | Stick swab | 1,388,557 |
| 74 | Bay (Beds 1–4) | Bathroom toilet bowl | 48 | Stick swab | 1,037,570 |
| 75 | Bay (Beds 1–4) | Bathroom toilet floor | 22 | Sponge swab | 581,158 |
| 84 | Bay (Beds 5–8) | Bay Medication Cupboard | 98 | Sponge swab | 1,597,559 |
| 97 | Bed 9 | Bathroom HWB Drain | 74 | Stick swab | 1,201,404 |
| 102 | Bed 9 | Bathroom toilet bowl | 118 | Stick swab | 2,036,935 |
| 142 | Bay (Beds 15–18) | Bathroom toilet bowl | 70 | Stick swab | 1,315,258 |
| 158 | Bay (Beds 21–24) | Bathroom toilet bowl | 145 | Stick swab | 2,429,559 |
| 176 | Staff toilet | Staff toilet HWB DWT | 290 | Wastewater | 4,156,799 |
| 177 | Staff toilet | Staff toilet HWB DWT | 75 | Sponge swab | 1,279,241 |
| 178 | Pantry (Staff & patient) | Sink DWT | 93 | Wastewater | 1,818,705 |
| 179 | Pantry (Staff & patient) | Sink DWT | 56 | Sponge swab | 1,284,616 |
| 182 | Room 33 | Office HWB DWT | 328 | Wastewater | 4,703,504 |
| 183 | Room 33 | Office HWB DWT | 177 | Sponge swab | 2,537,593 |
| 184 | Bay (Beds 1–4) | HWB DWT | 63 | Wastewater | 1,083,094 |
| 185 | Bay (Beds 1–4) | HWB DWT | 39 | Sponge swab | 654,450 |
| 186 | Bay (Beds 5–8) | HWB DWT | 440 | Wastewater | 6,622,675 |
| 187 | Bay (Beds 5–8) | HWB DWT | 126 | Sponge swab | 2,021,118 |
| 190 | Bed 9 | HWB DWT | 263 | Wastewater | 6,456,693 |
| 191 | Bed 9 | HWB DWT | 111 | Sponge swab | 2,078,635 |
| 194 | Bed 10 | HWB DWT | 51 | Wastewater | 795,058 |
| 195 | Bed 10 | HWB DWT | 88 | Sponge swab | 1,573,130 |
| 198 | Bay (Beds 15–18) | HWB DWT | 171 | Wastewater | 11,334,805 |
| 199 | Bay (Beds 15–18) | HWB DWT | 53 | Sponge swab | 9,700,533 |
| 202 | Bed 19 | HWB DWT | 160 | Wastewater | 2,521,155 |
| 203 | Bed 19 | HWB DWT | 41 | Sponge swab | 13,997,999 |
| 206 | Bay (Beds 29–32) | HWB DWT | 255 | Wastewater | 4,058,517 |
| 207 | Bay (Beds 29–32) | HWB DWT | 37 | Sponge swab | 789,087 |
| 208 | Bay (Beds 29–32) | Bathroom HWB DWT | 448 | Wastewater | 12,313,813 |
| 209 | Bay (Beds 29–32) | Bathroom HWB DWT | 14 | Sponge swab | 210,049 |
| 212 | Bed 20 | HWB DWT | 409 | Wastewater | 6,070,475 |
| 213 | Bed 20 | HWB DWT | 43 | Sponge swab | 30,494,541 |
HWB hand wash basin, DWT drain waste trap