TABLE 2.
Primers used in RT‐qPCR.
| Name | Primer sequence (5′–3′) | Tm (°C) | Product length ( bp) |
|---|---|---|---|
| GAPDH | F‐GACCTGCCGCCTGGAGAAAC | 60 | 120 |
| R‐AGAGTGAGTGTCGCTGTTGAAGTC | |||
| MYH11 | F‐AGCCAGAGACGAGAGGACCTTC | 60 | 120 |
| R‐AAGCCGTTGGAGAGGAATGTGTAG | |||
| TAGLN | F‐TCTTCAAGCAGATGGAGCAGGTG | 60 | 93 |
| R‐AGAGGTCAACGGTCTGGAACATATC | |||
| ACTA1 | F‐GCGTGGCTACTCCTTCGTGAC | 60 | 171 |
| R‐TTGCCGATGGTGATGACCTGAC | |||
| ACTG2 | F‐GCCACAGCAGCCTCCTCTTC | 60 | 191 |
| R‐TTGCGGATGTCAATGTCACACTTC | |||
| FLNC | F‐GGTGGAGGTGCTGTACGATGAC | 60 | 141 |
| R‐GTGAAGCGGTTAGCCTTGTTGAC | |||
| FLNA | F‐CGAGAAGCCACCACCGAGTTC | 60 | 196 |
| R‐CGTCGTAGGTCACATCCACAGAG |
Note: The melting temperature (Tm ) of each primer pair is 60°C, ensuring optimal annealing during PCR. Product length is specified for accurate amplification and subsequent analysis.