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Primer sequences
| Gene | Primer | Sequence (5′–3′) | Product size (bp) | Annealing temp (°C) | GenBank |
|---|---|---|---|---|---|
| METTL13 | Forward | CAGGAGGTTGATTACAGTGGC | 91 | 60.5 | NM_014955 |
| Reverse | CTCCATGACTCTAGCCGACA | ||||
| GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT | 197 | 61.6 | NM_001256799 |
| Reverse | GGCTGTTGTCATACTTCTCATGG |
