Fig. 4.

The role of NEAT1 in ER stress and apoptosis in GC. HGC27 cells were stably transfected with sh-NEAT1 for 72 h, and samples were collected after 48 h of tunicamycin treatment. A Volcano plot of the DEGs for NEAT1 differently expressed groups in TCGA-STAD, characterized as Log₂FC > 2 and adjusted P value < 0.05; B KEGG enrichment of the DEGs; C intersection of the DEGs for FUS, METTL3, and NEAT1 differently expressed groups; D western blotting analysis of UPR and apoptosis protein markers; E analysis of UPR markers with ImageJ; F analysis of apoptosis markers with ImageJ. N = 3 for western blotting. *P < 0.05 and **P < 0.01 by one-way ANOVA followed by Tukey’s multiple comparisons