Figure 4.

Cur inhibits the migration and invasion of MG63, U2OS cells. (A,B) Cell viability of MG63, U2OS cells treated with different concentrations of Cur for 24 h, respectively; (C) Morphological changes of cells under different concentrations (0 μM, 5 μM, 10 μM, 15 μM, 20 μM, 40 μM) of Cur treatment about 24 h, Bar=100 um;(D) Wound healing distance of MG63, U2OS cells after 24 h of different concentrations Cur treatment;(E, F). Relative quantification of wound healing distance in Fig. 4D; (G) The number of cells transferred into lower chamber after 24 h of Cur treatment, Bar=50 um; (H, I) Relative quantification of cell number in Fig. 4G; (G) The number of cells invaded into lower chamber after 24 h of Cur treatment (0 μM, 10 μM, 20 μM), Bar=50 um; (K. L) Relative quantification of cell number in Fig. 4J. (M) F-actin labeled skeletal changes in cells treated with Cur (10 μM) treatment, Bar=20 um; (N) Golgi reorientation ability in Ctrl, Cur treatment group during migration, respectively, Bar=25 um.