Figure 7.

Cur inhibits metastasis of OS dependent on EGFR/Src pathway. (A) In MG63 cells, EGFR intracellular location under Cur treatment or not, Bar=20 um; (B) Construction and identification of Ctrl, Src wild type (SRC-WT)and persistently active plasmid (Src-CA), Bar=20 um; (C) colocation of Exogenous p-Src (Tyr416) and Rab7-positive vesicles after Cur treatment or not in MG63 cells, Bar= 20 um; (D) Relative expression of protein level (PI3K, Akt1, p-Akt1, STAT3, ERK1/2, p-Src, Src, EGFR) in treat with Ctrl, Cur (10 μM), Over-expression of Src-CA and Cur (10 μM) + Over-expression of Src-CA; (E-K) Relative quantification of protein level in Fig 7D.