Table 1.
Bioprinting techniques | Classification | Printability | Cell viability | Ref |
---|---|---|---|---|
Extrusion-based bioprinting | Pneumatic-based | Bioink design (rheological properties, viscoelasticity, surface tension, gelation mechanism, etc.). Bioprinting process parameters pressure, speed, temperature, nozzle parameters, and crosslinking strategies). Construct design (filament spacing and orientation). |
Shear stress. Material cross-linking. Printing parameters (pressure, speed, nozzle size and shape). Characteristics of bio-ink. |
Fu et al., 75 Boularaoui et al. 76 |
Piston-driven | ||||
Screw-driven | ||||
Jetting-based bioprinting | Inkjet-based bioprinting | Highly dependent on the properties of the bioink. Two important parameters (Reynolds number/Weber number—determining the ink viscosity). The influence of pressure parameters on printability. The influence of nozzle parameters on printability (nozzle size and diameter). The influence of temperature on printability. |
Droplets (impact velocity, volume). Properties of bioink (Reynolds (Re) and Weber (We) numbers). Printing parameters (printing frequency, distance from nozzle to substrate). Shear stress. |
Ng and Shkolnikov, 77 Ng et al.78,79 |
Laser-assisted bioprinting | ||||
Acoustic-based bioprinting | ||||
Microvalve-based bioprinting | ||||
Electrohydrodynamic jet bioprinting | ||||
Light-based bioprinting techniques | Digital light processing, DLP | Types of photoinitiators. Wavelength and intensity of light. Composition of bio-ink. Structural resolution. |
Bioink composition (whether there are any additional protective components that can protect cells from light-induced damage). | Levato et al., 80 Fang et al., 81 Garciamendez-Mijares et al. 82 |
Stereolithography, SLA | ||||
Two-photon polymerization, 2PP |