Fig. 2.

SIRT6 deficiency promoted BLCA cell metastasis abilities in vitro and in vivo. A-C Wound healing assay of SIRT6-KD T24 (A) and UMUC-3 cells (B) and quantification analysis (C). Scale bar, 100 µm. D Wound healing assay and quantification analysis in control and SIRT6-overexpressing 253J or HT-1376 cells. E Quantification of transwell assays for SIRT6-KD T24 and UMUC-3 cells. Cell number was counted in six randomly captured pictures. F Representative bioluminescence (BIL) pictures showing the metastatic lesions in mice injected with control or SIRT6-KD UMUC-3 cells individually. G-H The BIL signals and metastatic lung lesions were quantified, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significance