Fig. 7.

PTEN is a direct target of miR-205-5p. A. The targets of miR-205-5p were predicted using four different miRNA target databases (miRanda, TargetScan, miRBase, and miRDB). B. qRT‒PCR results verified that PTEN was most downregulated among these genes. C. The prediction of binding sites between miR-205-5p and PTEN. D-E. The combination of PTEN and miR-205-5p was detected by dual-luciferase reporter assay. F. Quantitation of PTEN mRNA expression in the cells of each group after treatment with miR-205-5p mimics or inhibitors or their corresponding controls as measured by qRT‒PCR. G. PTEN protein expression in cells of each group after treatment with miR-205-5p mimics or inhibitors or their corresponding controls as detected by Western blotting. H. Semiquantitative analysis of PTEN protein levels. *P < 0.05 for all figures, n = 3 for each group