Figure 5.

Ciprofol suppressed H/R-induced ferroptosis and increased HIF-1α expression in H9c2 cardiomyocytes. Ciprofol (10 μM) was added 1 h before H/R. (A and B) Representative images and fluorescence intensity of FerroOrange staining. Scale bar = 50 µm. (C and D) Representative images and fluorescence intensity of Liperfluo staining. Scale bar = 50 µm. (E and F) Levels of Fe²⁺ and MDA. (G–J) Western blot bands and quantification of HIF-1α, ACSL4, and GPX4 protein expression. Data are shown as mean ± SD (n = 4‒6). *P < 0.05, **P < 0.01, ***P < 0.001.