Figure 9.

Ciprofol inhibited H/R-induced ferroptosis in H9c2 cardiomyocytes by upregulating HIF-1α. si-HIF-1α or si-NC was transfected 36 h before ciprofol (10μM) treatment. (A–C) Levels of Fe²⁺, MDA, and GSH. (D and E) Representative images and fluorescence intensity of FerroOrange staining. Scale bar = 50 µm. (F and G) Representative images and fluorescence intensity of Liperfluo staining. Scale bar = 50 µm. (H‒K) Western blot bands and quantification of HIF-1α, ACSL4, and GPX4 protein expression. Data are shown as mean ± SD (n = 4‒6). *P < 0.05, **P < 0.01, ***P < 0.001.