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. 2024 Sep 10;197(1):kiae476. doi: 10.1093/plphys/kiae476

Figure 4.

Figure 4.

Higher order rel2-relk mutants show defects in SAM maintenance during embryogenesis. A) Defects in SAM formation begin as early as 10 DAP in rel2;relk2;relk3 with defects in maintenance extending 14 DAP and 21 DAP. While SAM maintenance defects were detected in relk2;relk3 10 DAP, double mutants were able to recover SAM activity. By 10 DAP, rel2 formed a SAM and two leaf primordia similar to A619 and carried out normal embryo development 14 DAP and 21 DAP analogous to A619. n = 2–3 per genotype, except 21 DAP rel2;relk2;relk3 n = 1. Scale bars, 100 μm. B–D) To explore whether loss of apical activity was unique to rel2;relk2;relk3, C)rel2;relk1;relk3, and D)relk1;relk2;relk3 triple mutants were developed. Whereas by 20–25 days, B) A619 has formed a series of leaves, apical activity in the triple mutants terminates after the formation of two leaves similar to rel2;relk2;relk3. Scale bar = 1 cm.