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. 2024 Sep 10;197(1):kiae476. doi: 10.1093/plphys/kiae476

Figure 5.

Figure 5.

The rel2;relk1 ears are fasciated. A) SEM images of A619, relk1, rel2, and rel2;relk1 ear primordia. rel2 ears have an increase in width B) leading to a cylindrically shaped IM, which becomes enhanced into a tongue shape in rel2;relk1. In A) SEM images were digitally extracted for comparison. C)rel2 and rel2;relk1 mutants do not affect IM height. D) Heatmap of rel2;relk1 DEGs in RNA-seq data (scale = LogFC) revealed misregulation of peroxidases (PRXs) and superoxide dismutases (SODs), which suggested an IM environment locally high in O2−, which was confirmed by stronger NBT blue staining of F)rel2;relk1 relative to E) A619 ear primordia. Black dotted lines were over imposed to highlight the IM contour. n = 5 per genotype. Quantification by two-tailed Student's t-test. Box plot center line corresponds to median; box limits, upper and lower quantiles; whiskers, maximum and minimum values; ns = nonsignificant (P > 0.05), * = P ≤ 0.05, ** = P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Scale bars = 200 mm.