Table 1.
Human placental scRNA-seq studies in a normal pregnancy.
| Reference | Gestational age (weeks) | Population | Samples | Number of cells | Single cell isolation | Platform | Average reads length (bp) | Mean pair-end reads | Data repository |
|---|---|---|---|---|---|---|---|---|---|
| Pavlicev (1) | Term pregnancy | American | Two placentas from C-section without labor | 87 | Enzyme, gradient centrifugation | Fluidig C1 and Laser microdissection | 75 | 3.65 × 106 | GEO: GSE87726 |
| Vento-Tormo R (9) | 6-14 weeks | British | 11 deciduae and 5 placentas | 70,000 | Enzyme, gradient centrifugation, FACS | 10× Genomics v2, Smart-seq2 | 75 | 1 × 106 | ArrayExpress: E-MTAB-6701, E-MTAB-6678, and E-MTAB-7304 |
| Liu (20) | 8 weeks and 24 weeks | Chinese | 8 placentas | 1,471 | Enzyme, gradient centrifugation, MACS | Smart-seq2 | 150 | 1 × 106 | GEO: GSE89497 |
| Suryawanshi (11) | 6-11 weeks | American | 8 villi and 6 deciduae | 14,341, 6,754 | Enzyme | 10× Genomics and Drop-seq | 101 | N/A | BioProject: PRJNA492324 |
| Sun (65) | 11-13 weeks | American | 10 placental tissues | 7,245 | Enzyme | 10× Genomics | 2 × 75 | 1.901 ×107 | GEO: GSE131696 |
| Pique-Regi (60) | The second and third trimesters | American | 32 placental villi or decidua samples | 26,501 | Enzyme, gradient centrifugation | 10× Genomics 3’ | N/A | N/A | NIH dbGAP: phs001886.v2. p1 |
| Shannon (21) | 5-12 weeks | Canadian | 9 placental tissues | 50,790 | N/A | 10× Genomics | 50,000 | N/A | ArrayExpress: E-MTAB-6701 |
| Wang (85) | Full-term pregnancy | Chinese | 8 placentas | 11,438 | N/A | 10× Genomics | N/A | N/A | CNSA: CNP0000878 |
| Li (86) | 6-16 weeks | Chinese | 11 placentas | 52,179 | N/A | 10× Genomics | N/A | 150 | GSA: HRA003309 |
FACS, fluorescence-activated cell sorting; MACS, magnetic activated cell sorting; N/A, not available.