Figure 6.

Characterization of bacteriophage susceptibility to pH and temperature conditions. (A,B) Show the results of phage stability at different temperatures and pH conditions, respectively. In general, all three bacteriophages were stable at temperatures between 4°C and 50°C. A decrease of 3.9 Log was observed in the FKP3 bacteriophage at a temperature of 70°C (ANOVA, p = 4.22×10⁻¹¹ ***). A decrease of 2.8 Log at 60°C was observed in phage FKP10 (Kruskal-Wallis, Dunnet test adjusted with the Bonferroni method; p = 1.0) and 6.6 Log at 70°C (Kruskal-Wallis, Dunnet test adjusted with Bonferroni method; p = 0.258). All three bacteriophages were stable at pH 4–10 (FKP3, Kruskal-Wallis p = 0.849; FKP10, ANOVA p = 0.700 and FKP14, Kruskal-Wallis p = 0.957), whereas they lost their stability at pH 2 and 12.