Figure 3.

Probiotic potential of L. reuteri strains in various conditions. (A) The survival of L. reuteri strains was tested in simulated gastric conditions at pH 2 for 2 h, comparing their viability to the reference strain L. reuteri ATCC53608. (B) The viability of L. reuteri strains in MRS media at pH 2 for 2 h was measured. (A,B) One-way ANOVA was used for statistical comparison against the reference strain. (C) The viability of L. reuteri strains in bile salts at 0.1, 0.2, and 0.3% for 2 h. Statistical difference was determined against the reference strain using two-way ANOVA. (D) The viability of L. reuteri strains in a simulated colonic environment at neutral pH over 12 and 24 h. Statistical difference was determined against the reference strain using two-way ANOVA. (E) Competition assay to measure the inhibitory effect of L. reuteri strains on the growth of ETEC K88 after 24 h of co-culture. Statistical difference was determined against the reference strain using one-way ANOVA. (A–E) Significant differences are marked by asterisks for p-values less than 0.05.