Figure 6.

Random forest (RF) model assisted health index prediction. (A) Ranking of health-associated bacterial taxa. The importance scores of input taxa were evaluated by the RF model, with the top 30 important taxa presented. (B) Health index predictions by the RF model. Healthy calves (HC) typically exhibited a health index of around 1, while diarrheic calves (DC) primarily showed a Health Index below 0.5. (C) Changes of health index during in vivo treatment. The microbiota data was utilized in the RF model, and the health index was indicated for each day throughout the treatment course. Overall, the health index scores of all three calves with probiotic treatment demonstrated progressive improvement in gut health status over the observed period.