Figure 7.

Determining the Modulation of Deacetylated-Sialic Acid to Selectin Binding via Sialylglycan Recognition Probes (SGRP). The binding of L-Selectins (A-C) and P-Selectins (D-F) to control cell lines A549, HCT 116, and HEK 293, respectively, were evaluated following treatment with an esterase that specifically removes sialic acid acetyl groups. Cells were seeded in 96-well V-bottom plates, washed with FACS buffer, and treated with 9-O-Ac probe (PTOV He-Fc, #1554). After a 1.5-hour incubation at 37°C, cells were washed again and incubated with Selectins-FITC conjugates at 4°C for 1 hour in the dark. Data were acquired using flow cytometry. For statistical analysis was conducted using Two-way ANOVA to analyze the data of these experiments for comparison using GraphPad Prism 8. Data, as seen below, is always presented as mean ± standard deviation with P < 0.05 indicating significance. Error bars represent the standard deviation of triplicate samples. ** p<0.005, ****p<0.0001, ns = not significant (p>0.05).