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. 2024 Dec 17;16:2063–2082. doi: 10.2147/NSS.S485952

Figure 2.

Figure 2

IH promotes pyroptosis and inflammation of bEnd3 cells (n=3). (A and B) The expression of GSDMD and Caspase-1 protein and mRNA in bEnd3 cells was detected by Western blot and qRT-PCR. (C) The levels of IL-1β, IL-6, IL-18, and TNF-α in the supernatant of bEnd3 cells were detected by ELISA. (D) The expression of miR-20a-5p in bEnd3 cells was detected by qRT-PCR. Statistical analysis of the data was performed using Student’s t-test, df=4. *P<0.05.