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. 2024 Dec 17;16:2063–2082. doi: 10.2147/NSS.S485952

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© 2024 Chen et al.

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The exosomes secrete miR-20a-5p (n=3). (A) The expression of miR-20a-5p in exosomes was detected by qRT-PCR. (B) Exosomes isolated from bEnd3 cells transfected with Cy3-labeled miR-20a-5p mimics (red) were co-cultured with HT22 cells. (C) Fluorescence images of HT22 cells incubated with exosomes derived from bEnd3 cells transfected with Cy3-miR-20a-5p. Magnification×200. Scale bar, 50 µm. Magnification×400. Scale bar, 20 µm. (D) The expression of miR-20a-5p in HT22 cells was detected by qRT-PCR. Statistical analysis of the data was performed using Student’s t-test ((A), df=4), one-way ANOVA (D) followed by Bonferroni’s multiple comparison tests, df (2, 6). *P<0.05.