Figure 11.

miR-20a-5p promotes pyroptosis of HT22 cells by mediating IH-EXOS by targeting MFN2 (n=3). (A and E) qRT-PCR was used to detect the expression of miR-20a-5p in miR-20a-5p silencing or overexpression with or without IH-EXOS exposure. (B, C, F and G) Western blot and qRT-PCR were used to detect the expression of MFN2, GSDMD, and Caspase-1 protein and mRNA in miR-20a-5p silencing or overexpression with or without IH-EXOS exposure. (D and H) ELISA was used to detect IL-1β, IL-6, IL-18, and TNF-α levels in miR-20a-5p silencing or overexpression with or without IH-EXOS exposure. (I) Prediction of interactions between miR-21-3p and its putative binding sites in the 3’UTR of MFN2. (J) After co-transfection of miR-20a-5p mimics and MFN2 WT or MUT reporter plasmids for 48h, luciferase activity in HEK293T cells was evaluated and normalized. Statistical analysis of the data was performed using two-way ANOVA followed by Bonferroni’s multiple comparison tests, df (2, 6). *P<0.05.

Abbreviations: WT, wild type; MUT, mutant type.