Fig. 2.
Selective enrichment of plant-based meat analogues (PBMAs)-associated bacteria from control (A) and E. coli O157:H7-contaminated (B, C) samples throughout a two-step cultural procedure. Retail PBMA samples (n = 6) pre-inoculated with different levels of E. coli O157:H7 in duplication were subjected to bacterial enrichment by first culturing in modified Tryptone Soya Broth supplemented with novobiocin (mTSB-n) at 42oC for 24 h, followed by passaging the primary enrichment products into Enterohemorrhagic E. coli Enrichment Broth (EEB) with further incubation at 35oC for 24 h. Differential abundance analysis ANCOM-BC2 was conducted to identify bacterial genera that were significantly enriched after the primary (red, mTSB-n) and secondary (blue, EEB) culturing. Horizontal bars represent, in log-scale (natural log), fold change ± standard error (shown as error bars) of absolute abundance associated with the primary- and secondary-enrichment cultures relative to the pre-enriched reference samples. Only taxa with statistically significant change in their abundance relative to the reference group were displayed.