Fig. 3.

Deletion of Notch1 in macrophages ameliorates HFD-induced hepatic steatosis and insulin resistance.

Notch1^FL/FL mice and Notch1^M-KO mice were fed a NCD or HFD for 6 weeks. GTT (A), ITT (B), serum TG, TC, LDL-C, and HDL-C levels (C), and liver TG and TC levels (D) were measured (eight/group). (E) Representative images of H&E and Oil red O staining of liver sections (scale bar: 100 μm). (F) Western blot analyses of insulin-stimulated expression of Akt and pAkt in the liver (three/group). Values represent means ± SD. Statistical analysis was performed by one-way ANOVA and Tukey's test: ∗p <0.05, ∗∗p <0.01, ∗∗∗p <0.001. Abbreviations: GTT, glucose tolerance test; HDL-C, HDL cholesterol; HFD, high-fat diet; ITT, insulin tolerance test; LDL-C, LDL cholesterol; NCD, normal chow diet; Notch1^FL/FL, floxed Notch1; Notch1^M-KO, myeloid-specific Notch1-knockout; TC, total cholesterol; TG, triglyceride.