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Analysis of FGFR3-TACC3 mRNA expression in bladder cancer cells by qRT-PCR. RT4 and RT112 were transfected with CRISPR-Cas13a (#1, #2, #3) or Cas13a-NC and shRNA (#1, #2) or shRNA-NC for 24 h. (A and B). mRNA expression of FGFR3-TACC3 was reduced after knockdown of FGFR3-TACC3 by CRISPR-Cas13a and shRNA in RT4 and RT112 cells. The Cr2 and Si1 groups with more effective knockdown were selected for subsequent experiments. Significant results are presented as ***p < 0.001, ###p < 0.001.
