Fig. 2.

Ovulatory FF induces two waves of AID expression in the FTE in accordance with Early and Late exposure after ovulation. (a) IHC staining of AID in oviduct sections of wild-type (WT) and progesterone knockout (Pgr KO) mice with or without superovulation. (b) The AID mRNA expression of FE25 cells treated with 100 % FF (2 min, washed) checked at different points from 0 min to 1 h and with 10 % FF (no wash) checked from 2–8 h. IHC staining of AID in human fallopian tube fimbria ex vivo after exposure to vehicle, 100 % FF (2 min, washed) checked at different time intervals from 30 min to 1 h (c), and 10 % FF (no wash) checked from 7–9 h (d). * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed paired Student's t-test.