Fig. 4.
AID is required for mutagenesis by FF-ROS. (a) Confirmation of AID knockdown in FE25 cells by western blot analysis. (b & c) Representative images and graphical representation of HPRT colonies and mutation frequency in FE25 cells with or without AID knockdown exposed to 10 % FF and H2O2 (100 μM). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the same untreated cells; # p < 0.05, # # p < 0.01, # # # p < 0.001 comparison of treatment groups in FE25 cells and FE25 shAID1 & FE25 shAID2 cells by two-tailed paired Student's t-test.