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. 2024 Dec 13;151(24):dev202549. doi: 10.1242/dev.202549

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© 2024. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Germ cells are retinoic acid responsive during early gonad development. (A-H) Representative confocal images of 12 (A-D) and 23 (E-H) dpf wild-type gonads in Tg(RARE:YFP) transgenics. (I-L) Representative confocal images of a 13 dpf wild-type gonad from a Tg(piwil1:EGFP) transgenic stained using anti-Crabp2a antibody. (A,E) YFP-labeled RA-responsive cells are green; anti-Ddx4 antibody labeled germ cells (GCs) are red. (I) EGFP-labeled piwil1⁺ GCs are green; Crabp2a-expressing cells are red. (A,E,I) DAPI-labeled nuclei are blue. (B,F) Grayscale Ddx4, (C,G) YFP, (J) EGFP, (K) Crabp2a and (D,H,L) DAPI. (A-D,I-L) Z-projections. (E-H) Single slices. Scale bars: 50 μm. Wild type: 12 dpf, n=3 larvae; 23 dpf, n=1 fish; 13 dpf, n=1 fish. Both gonads from each fish were imaged.