Table 2.
Amino acids | Concentration (mg/100 g) |
|||||
---|---|---|---|---|---|---|
T0 | T1 | T2 | T3 | T4 | T5 | |
Aliphatic amino acid | 558.79 ± 0.95d | 603.83 ± 17.36c | 622.24 ± 5.55c | 692.98 ± 8.66b | 694.22 ± 12.35b | 918.42 ± 8.88a |
Thr | 5.74 ± 0.19 bc | 8.36 ± 2.28 a | 6.84 ± 0.24 ab | 7.94 ± 0.13 a | 6.89 ± 0.19 ab | 4.25 ± 0.34 c |
Val | 4.44 ± 0.09 e | 4.60 ± 0.04 e | 6.89 ± 0.19 d | 9.77 ± 0.51 c | 14.21 ± 0.67 b | 37.40 ± 0.88 a |
Met | 1.44 ± 0.21 e | 1.85 ± 0.06 e | 3.36 ± 0.06 d | 7.85 ± 0.80 c | 11.75 ± 0.79 b | 21.12 ± 0.85 a |
Leu | 4.87 ± 0.14 e | 4.10 ± 0.34 e | 8.83 ± 1.09 d | 14.71 ± 0.62 c | 22.69 ± 0.68 b | 61.61 ± 0.86 a |
Lys | 31.00 ± 1.23 b | 29.65 ± 1.25 b | 36.20 ± 0.69 a | 25.26 ± 0.80 c | 12.06 ± 0.81 d | 9.15 ± 0.34 e |
Ile | 7.20 ± 0.15 c | 12.38 ± 0.90 b | 16.62 ± 0.07 a | 15.46 ± 1.35 a | 5.37 ± 1.63 c | 2.67 ± 0.43 f |
Glu | 12.27 ± 0.80 b | 11.53 ± 0.24 b | 19.66 ± 0.74 a | nd | nd | nd |
Asn | nd | nd | nd | 55.85 ± 0.55 c | 86.05 ± 1.18 b | 107.38 ± 6.25 a |
Gln | 113.15 ± 3.54 d | 133.77 ± 7.66 c | 127.04 ± 4.76 c | 148.45 ± 3.13 b | 154.32 ± 5.53 b | 215.45 ± 1.76 a |
Arg | 11.39 ± 0.37 f | 80.22 ± 0.62 e | 106.77 ± 0.78 d | 138.14 ± 1.95 c | 173.52 ± 1.09 b | 267.74 ± 0.53 a |
Ala | 19.01 ± 0.73 a | nd | nd | nd | nd | nd |
Tau | 261.72 ± 0.79 a | 245.08 ± 3.67 b | 210.68 ± 2.18 d | 238.10 ± 2.63 c | 207.35 ± 3.91 d | 191.65 ± 2.58 e |
Gly | 86.56 ± 1.36 a | 72.29 ± 1.54 c | 79.37 ± 0.90 b | 31.45 ± 0.63 d | nd | nd |
Aromatic amino acid | 31.52 ± 0.60f | 38.02 ± 1.28e | 44.91 ± 0.70d | 126.21 ± 1.09c | 155.32 ± 1.63b | 212.86 ± 0.91a |
Tyr | 2.39 ± 0.26 e | 2.62 ± 0.14 e | 4.26 ± 0.03 d | 7.91 ± 0.15 a | 7.47 ± 0.17 b | 4.96 ± 0.15 c |
Trp | 29.13 ± 0.72 f | 35.41 ± 1.17 e | 40.65 ± 0.72 d | 118.30 ± 1.23 c | 147.84 ± 1.71 b | 207.89 ± 0.88 a |
Heterocyclic amino acid | 26.25 ± 0.49b | 6.15 ± 0.64e | 40.99 ± 0.11a | 24.57 ± 0.80c | 17.31 ± 0.56d | nd |
Pro | 20.75 ± 0.52 c | nd | 37.27 ± 0.14 a | 22.34 ± 0.67 b | 15.46 ± 0.41 d | nd |
His | 5.50 ± 0.21 a | 6.15 ± 0.64 a | 3.72 ± 0.24 b | 2.23 ± 0.16 c | 1.85 ± 0.15 c | nd |
∑FAA | 616.56 ± 0.94 f | 648.00 ± 19.11 e | 708.14 ± 6.06 d | 843.76 ± 8.47 c | 866.84 ± 13.16 b | 1131.28 ± 8.44 a |
Different letters represented significant difference (P < 0.05); nd means not detected.