Skip to main content
. 2024 Dec 6;14:1433256. doi: 10.3389/fonc.2024.1433256

Figure 4.

Figure 4

Effect of ERK1/2 signaling on glutathione and cystine transport. (A) Intracellular concentrations of GSH after ERK1/2 activation. Significant 1,3.-fold (p<0.01), 1,11-fold (p=0.04) and 2,19-fold (p=0.02) differences in GSH levels were observed in NBL-S, SK-N-DZ, and SK-N-SH cells, respectively. (B) Correlation of baseline cystine-FITC transport in the presence of phospho-ERK1/2 demonstrated that cells with higher levels of phospho-ERK1/2 exhibited higher baseline cystine transport. (C) Cystine transport, measured by cystine-FITC in SK-N-SH pmRNA5-ctrl or pmRNA5-caMEK cells, demonstrated a 2.44-fold (p=0.042) increase. Western blot demonstrates an increase in ERK1/2 and SLC7A11 phosphorylation in caMEK SK-N-SH cells. (D) Cystine transport, measured by cystine-FITC, increased 1.86-fold (p=0.037) and decreased 1.57-fold (p=0.042) in KCNR and NBL-S cells, respectively, after 6 h of pretreatment with 10 μM SCH772984. Western blot shows the respective increase and decrease in SLC7A11 phosphorylation after pretreatment with 10 μM SCH772984. *<0.05.