Fig. 1.

Decreased neutrophil autophagy in the peripheral blood of asthma patients. A) The contents of IL-4, IL-8 and IL-17 in serum were measured using ELISA. B) Flow cytometry was utilized to measure the percentage of neutrophils in peripheral blood. C) Expression of neutrophil activity markers CD11a, CD11b and CD18 was detected using RT-qPCR. *p < 0.05 vs. Healthy and #p < 0.05 vs. Mild asthma D) Expression of autophagy marker LC3 in neutrophils was detected by IF. (E) WB was used to analyze the protein expression of BECN1, LC3 and p62 in neutrophils. *p < 0.05 vs. Healthy and #p < 0.05 vs. Mild asthma E) WB was used to analyze the protein expression of BECN1, LC3 and p62 in neutrophils. *p < 0.05 vs. Healthy and #p < 0.05 vs. Mild asthma