Fig. 4.

SHP2 inhibited autophagy in neutrophil-like cells. A) Cellular polarization morphology changes were observed through Giemsa staining. B) CD11b expression in cells after 6 days of DMSO treatment was detected by RT-qPCR. *p < 0.05 vs. HL-60. C, D) SHP2 expression level was analyzed in neutrophil-like cells by RT-qPCR and WB. E) CCK-8 assay was utilized to detect cell viability. *p < 0.05 vs. Control and #p < 0.05 vs. RAP + oe-NC F) Distribution of LC3 in neutrophil-like cells was detected using IF. G) Protein expression levels of BECN1, LC3 and p62 were detected in neutrophil-like cells using WB. *p < 0.05 vs. Control and #p < 0.05 vs. RAP + oe-NC