Fig. 5.

Inhibition of NET formation in neutrophil-like cells through down-regulating the SHP2/ERK5 pathway. A) RT-qPCR and WB were performed to detect SHP2 expression. *p < 0.05 vs. si-NC and #p < 0.05 vs. si-SHP2 + oe-NC B) IF staining was employed to detect cit-H3 expression. C, D) Levels of dsDNA and MPO activity in neutrophil-like cells were measured. *p < 0.05 vs. si-NC and #p < 0.05 vs. si-SHP2 + oe-NC E, F) Protein expression of MPO, ELANE, PADI4 and ERK5 was analyzed using WB. G) Expression levels of SHP2 and ERK5 were analyzed using RT-qPCR and WB. *p < 0.05 vs. si-NC and #p < 0.05 vs. si-SHP2 + oe-NC H) Cit-H3 expression was detected using IF. I, J) Levels of dsDNA and MPO activity in neutrophils were measured. K) WB was performed to analyze the protein expression of MPO, ELANE, and PADI4 in neutrophil-like cells. *p < 0.05 vs. si-NC and #p < 0.05 vs. si-SHP2 + oe-NC