Fig. 6.

Down-regulation of SHP2 promoted autophagy and inhibited formation of NETs in peripheral blood-derived neutrophils from asthma patients. A) Expression levels of SHP2 and ERK5 were detected by RT-qPCR and WB in neutrophils. B) IF and WB were utilized to detect the expression of LC3, BECN1 and p62 to assess neutrophil autophagy. *p < 0.05 vs. si-NC C) IF and WB were utilized to detect the expression of LC3, BECN1 and p62 to assess neutrophil autophagy. D) Expression of cit-H3 was detected using IF. *p < 0.05 vs. si-NC E, F) Levels of dsDNA and MPO activity in neutrophils were measured by ELISA. G) WB was performed to analyze the protein expression of MPO, ELANE, and PADI4 in neutrophils. *p < 0.05 vs. si-NC