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. 2024 Dec 23;30:269. doi: 10.1186/s10020-024-01047-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ADAM19 affects the expression of profibrotic cytokines NRG1 in human dermal fibroblasts. (a) Heatmap displaying ADAM19 and genes involved in wound healing pathway expression in human primary dermal fibroblasts isolated from HC transfected with ADAM19 siRNA with or without TGF-β (10ng/ml) stimulation for 48 h. (b) mRNA expression of NRG1 in isolated primary dermal fibroblasts from HC and SSc patients. (c) mRNA expression of COL1A1, ACTA2, and pro-inflammatory cytokine IL-6 in recombinant human NRG1 (50ng/ml) incubation with human primary dermal fibroblast isolated from HC 24 h. (d) mRNA and protein levels of NRG1 in human primary dermal fibroblasts isolated from HC transfected with negative control siRNA and ADAM19 siRNA with or without TGF-β (10ng/ml) stimulation for 48 h. (e) The expression of sNRG1 in the culture supernatant of human primary dermal fibroblasts isolated from HC transfected with negative control siRNA and ADAM19 siRNA with or without TGF-β (10ng/ml) stimulation for 48 h. (f) mRNA expression of ADAM19 and ACTA2 in human primary dermal fibroblasts isolated from HC transfected with negative control siRNA and ADAM19 siRNA, and then stimulated with or without TGF-β (10ng/ml) and recombinant human NRG1 (50ng/ml) stimulation. HC, healthy controls; SSc, systemic sclerosis. Data are represented as mean ± SEM. *, P < 0.05, **, P < 0.01