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. 2024 Nov 19;27(12):111417. doi: 10.1016/j.isci.2024.111417

Figure 5.

Figure 5

Cell-cell interactions between tip endothelial cells and FTSEC3

(A) EC3_Endo_ESM1 output signal pathway intensity. Left side indicates outgoing signaling patterns, right side represents incoming signaling patterns. Color represents relative cell communication strength, with darker colors indicating greater intensity.

(B) UMAP plot showing pan-cancer endothelial cells clusters by color.

(C) The violin plots show the expression of indicated genes in tip endothelial cells of 5 different cancer types.

(D) The circle plot shows the TGF-β signal pathway network. Colors represent different clusters of epithelial and endothelial cells. The thickness of the lines indicates the strength of cell communication. Thicker lines indicate greater communication intensity.

(E) The violin diagram shows the distribution of ligand-receptor gene expression in the TGF-β signaling pathway in different epithelial and endothelial clusters.

(F) Quantification of CRISPR knockout (KO) efficiency (indel %) in HUVECs (mean ± SD, n = 5).

(G) Quantification of spheroid-based in vitro sprouting angiogenesis (n = 10 spheroid per group, mean ± SD). ∗, adjusted p value <0.05; ∗∗∗, adjusted p value <0.001; ∗∗∗∗, adjusted p value <0.0001, one-way ANOVA.