REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CD31/PECAM-1 antibody (H-3)	Santa Cruz Biotechnology	sc-376764
anti-ESM1	Abcam	ab235966
goat anti-mouse Alexa Fluor 488	Invitrogen	A-11001
donkey anti-rabbit Alexa Fluor 647	Invitrogen	A-31573
Chemicals, peptides, and recombinant proteins
M199 medium	Gibco	Cat#22350-029
L-glutamine	Gibco	Cat#35050-061
Endothelial Cell Growth Supplement (ECGS)/Heparin	PromoCell	Cat#C-30120
fetal bovine serum	Sigma	Cat#F7524
SpCas9	IDT	Cat#1081059
Opti-MEM	Thermo Scientific	Cat#31985062
AccuPrime PFX Reaction Mix	Invitrogen	Cat#92008
methylcellulose	Merck	Cat#M05129
Collagen-type I solution	Merck	Cat#08-115
4% paraformaldehyde	AH Diagnostics	Cat#SC-281692
Critical commercial assays
NucleoSpin Gel and PCR Clean-up kit	Macherey	Cat#2006/001
Mix2Seq Kit	Eurofins Genomics	N/A
Experimental models: Cell lines
Human umbilical vein endothelial cells (HUVECs)	Isolated from human umbilical vein, this study	N/A
Oligonucleotides
LAMA4 gRNA (spacer sequences): TGACATTGAAGGGAGCTCAG	Synthego, US	N/A
TGFB1 gRNA (spacer sequences): GGCACCTCCCCCTGGCTCG	Synthego, US	N/A
TGFBR1 gRNA (spacer sequences): TTGACTTAATTCCTCGAGAT	Synthego, US	N/A
Software and algorithms
Seurat	https://satijalab.org/seurat/	version 4.1.0
cNMF	https://github.com/dylkot/cNMF	version 1.3.5
Monocle2	https://cole-trapnell-lab.github.io/monocle-release/	version 2.22.0
harmony	https://github.com/immunogenomics/harmony	version 0.1.0
org.Hs.eg.db	https://bioconductor.org/packages/release/data/annotation/html/org.Hs.eg.db.html	version 3.16.0
clusterProfiler	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html	version 4.6.0
ggplot2	https://ggplot2.tidyverse.org/	version 3.3.5
GSVA	https://bioconductor.org/packages/release/bioc/html/GSVA.html	version 1.46.0
pheatmap	https://www.rdocumentation.org/packages/pheatmap/versions/1.0.12/topics/pheatmap	version 1.0.12
PROGENy	https://github.com/saezlab/progeny	version 1.16.0
pyscenic	https://github.com/aertslab/pySCENIC	version 0.11.2
CellChat	https://github.com/sqjin/CellChat	version 1.5.0
CIBERSORTx	https://cibersortx.stanford.edu/	version 0.1.0
ImageJ	https://imagej.net/ij/	version 2.9.0
Code availability	https://github.com/lianglangchao/ovarian_cancer_llc_V2	N/A
Ovarian cancer single cell RNA expression database	https://dreamapp.biomed.au.dk/OvaryCancer_DB/	N/A
Integrated OC scRNA-seq data	https://lambrechtslab.sites.vib.be/en/pan-cancer-blueprint-tumor-microenvironment-0	N/A
Integrated OC scRNA-seq data	https://lambrechtslab.sites.vib.be/en/high-grade-serous-tubo-ovarian-cancer-refined-single-cell-rna-sequencing-specific-cell-subtypes	N/A
Integrated OC scRNA-seq data	https://github.com/vicDRC/CCR_CD39study/tree/master/data	N/A
Integrated OC scRNA-seq data	Gene Expression Omnibus (GEO)	GSE140819 GSE173682 GSE165897 GSE178101 GSE151214 GSE150443 GSE151316 GSE184880 GSE160755 GSE193371 GSE194105 GSE154600 GSE130000 GSE158937
Other
Nikon laser confocal microscope	Japan	A1 HD25