Table 1.
Biophysical properties of the assessed voltage gated channels from rat DRG neurons in the absence and presence of SGC-PIKFYVE-1.
| Condition | IV curve | Activation | Fast inactivation | |||||
|---|---|---|---|---|---|---|---|---|
|
Peak/density (pA/pF) |
n |
V0.5 (mV) |
k | n |
V0.5 (mV) |
k | n | |
| Total sodium currents + 0.1 % DMSO | −281.2 ± 32.24 | 15 | −21.776 ± 0.50 | 4.93 ± 0.44 | 15 | −53.6 ± 1.56 | −13.64 ± 1.53 | 15 |
| Total sodium currents + SGC-PIKFYVE-1 (1 µM) | −370.4 ± 46.81 | 20 | −21.09 ± 0.74 | 6.54 ± 0.66 | 20 | −51.69 ± 1.67 | −14.49 ± 1.69 | 20 |
| Total sodium currents + 0.1 % DMSO | −533.2 ± 42.99 | 21 | −20.48 ± 0.48 | 4.25 ± 0.42 | 21 | −47.42 ± 0.79 | −11.11 ± 0.74 | 21 |
| Total sodium currents + SGC-PIKFYVE-1 (10 µM) | −348.7 ± 38.42* | 11 | −20.11 ± 0.63 | 4.89 ± 0.56 | 11 | −51.51 ± 0.79* | −10.32 ± 0.73 | 11 |
| Total sodium currents + ProTxII (5 nM) | −207.3 ± 57.12* | 6 | −14.89 ± 0.57* | 4.17 ± 0.49 | 6 | −53.17 ± 1.24* | −11.11 ± 1.15 | 6 |
| Total sodium currents + SGC-PIKFYVE-1 (10 µM) + ProTxII (5 nM) | −268.8 ± 34.33 | 13 | −20.23 ± 0.48 | 4.00 ± 0.42 | 13 | −45.41 ± 1.28 | −12.06 ± 1.21 | 13 |
| Total sodium currents + 0.1 % DMSO | −358.2 ± 32.87 | 16 | −24.61 ± 0.44 | 3.70 ± 0.39 | 16 | −52.39 ± 1.14 | −12.25 ± 1.08 | 16 |
| Total sodium currents + SGC-PIKFYVE-1 (10 µM) | −189.7 ± 47.11* | 6 | −20.18 ± 0.55 | 3.73 ± 0.47 | 6 | −58.85 ± 1.79* | −10.25 ± 1.63 | 6 |
| Total sodium currents + VX-548 (5 nM) | −200.5 ± 35.37* | 11 | −19.51 ± 0.98* | 6.18 ± 0.88* | 11 | −59.85 ± 1.10* | −9.98 ± 0.99 | 11 |
| Total sodium currents + SGC-PIKFYVE-1 (10 µM) + VX-548 (5 nM) | −186.1 ± 26.63 | 14 | −21.43 ± 0.61* | 4.85 ± 0.53 | 14 | −59.37 ± 1.23* | −10.62 ± 1.12 | 14 |
| Total calcium currents + 0.1 % DMSO | −107.71 ± 14.20 | 20 | −4.68 ± 0.73 | 5.99 ± 0.64 | 20 | −36.91 ± 0.73 | 0.99 ± 0.01 | 20 |
| Total calcium currents + SGC-PIKFYVE-1 (10 µM) | −113.06 ± 13.24 | 20 | −4.31 ± 0.67 | 5.73 ± 0.58 | 20 | −34.76 ± 1.79 | 0.98 ± 0.02 | 20 |
| Total potassium currents + 0.1 % DMSO | 184.90 ± 17.17 | 15 | − | − | − | − | − | − |
| Total potassium currents + SGC-PIKFYVE-1 (10 µM) | 213.92 ± 21.52 | 12 | − | − | − | − | − | − |
Values are presented as means ± SEM. Gating properties were calculated from fits of the data obtained from the indicated number of individual cells to the Boltzmann equation. The values include V0.5, which represents the midpoint potential (mV) for voltage-dependent of activation or inactivation, and k, the slope factor. These values pertain to Fig.2, Fig.3, Fig.4. * p < 0.05 with respect to 0.1 % DMSO.