Fig. 3. (A) and (B) Fluorescence spectra of FL-G41C CaM (A) and FL-G114C CaM (B) before and after mixing with Adgb. The arrows indicate the decrease in the intensity. (C) The docking structure of the Adgb–CaM complex with the lowest binding energy calculated by HDOCK. The chemical modification of Cys41 by FL is shown to clarify the energy transfer to the heme group, as indicated by the arrow. (D) The predicted structure of the Adgb–CaM complex by AlphaFold3. The position of heme binding is highlighted.
