Fig. 3.

The biocompatibility and ROS scavenging properties of CeMn-PEG in vitro. (A and B) Effects of various concentrations of CeMn-PEG (0, 0.25, 0.5, 1, and 2 μg/ml) on the viability of both NP and RAW264.7 cells after incubation for 24 h by using CCK8 assay. (C to F) Representative ROS fluorescence microscopy images and relative fluorescence intensity of NP cells (C and E) and RAW264.7 cells (D and F) pretreated with various concentrations of CeMn-PEG (0, 0.25, 0.5, 1, and 2 μg/ml) after the treatment of H₂O₂ (200 μM) for 6 h. Scale bar, 25 μm. Data are presented as the mean ± SEM. ^#P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 relative to the control group; ^P > 0.05, ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 relative to the H₂O₂-treated group, n = 3.