Fig. 4.

CeMn-PEG ameliorated H₂O₂-induced inflammation and ECM degradation and reduced H₂O₂-induced apoptosis and autophagy in vitro. (A) The relative mRNA expression of IL-1β, IL-4, IL-6, IL-10, collagen II, aggrecan, MMP13, and ADAMTS5 was determined by using qPCR. (B and C) Representative images and quantification data of Western blot results of IL-1β, IL-4, IL-6, IL-10, collagen II, aggrecan, MMP13, and ADAMTS5. (D and E) Representative images and relative fluorescence intensity of double immunofluorescence of LC3 (red) and C-caspase3 (green) in NP cells. (F) Representative images and quantification data of Western blot results of LC3, p62, and C-caspase3. Scale bar, 25 μm. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3.