Fig. 5.
RAW264.7 cells pretreated with CeMn-PEG could alleviate H2O2-induced ECM degradation and apoptosis in NP cells. (A) Diagram of RAW264.7 cells (apical chamber) and NP cells (basal chamber) cocultured by using Transwell dish. RAW264.7 cells were pretreated with CeMn-PEG and then cocultured with NP cells. (B and C) Representative images and quantification data of Western blot results of collagen II, aggrecan, MMP13, and ADAMTS5 in NP cells in the coculture system. (D) Representative images of flow cytometry analysis of NP cells in the coculture system. (E) Apoptosis rate of NP cells in the coculture system estimated by flow cytometry analysis. (F and G) Representative images and relative fluorescence intensity of C-caspase3 (green) in NP cells in the coculture system. Scale bar, 25 μm. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3.