Table 1. Properties of DNA molecules used.

DNA	N, bp*	Lc, μm†	Rg, nm‡	Nb,§	τ1, ms¶	Δt, ms∥
φX174**	5,386	1.8	175	1.4	2.6	21
pΔ	8,000	2.7	213	2.0	4.8	21
T7/BstEII	20,065/19,871††	6.8	336	5.0	19	26
T7	39,936	13.6	476	10.1	53	27
T4	166,000	56.4	970	41.8	450	52

^*Degree of polymerization.

^†Contour length; L_c = 0.34 nm·N.

^‡Radius of gyration; R_g = (PL_c/3)^1/2 with a persistence length of P = 50 nm.

^§Number of DNA blobs in nanotube of radius R = 150 nm. N_b = N/n, where n = 3,970 is the number of bp per blob in a cylindrical tube, obtained from the condition that the blob radius-of-gyration equals the tube radius.

^¶Longest relaxation time of unconfined polymer. Confinement will increase the relaxation time as described in the text.

^∥Experimental average transit time across detection volume.

^**Linear form obtained by restriction cleavage of circular form by Eco 1471. The uncleaved sample contained >90% nicked circular form.

^††Two fragments in equimolar amounts obtained by restriction cleavage of T7 DNA by BstEII. Calculations and plots are based on the mean N.