Fig. 3.

The ADAM10 inhibitor GI254023X rescues E-cadherin cell surface expression and β-catenin translocation after ionomycin treatment. HaCaT cells were mock-treated (A, D, and G) or stimulated with ionomycin (5 μM) for 30 min in the presence (C, F, and I) or absence (B, E, and H) of the ADAM10 inhibitor GI254023X (GI, 5 μM). Afterward, cells were fixed with PFA and immunostained with N-terminal (green) and C-terminal (red) E-cadherin and β-catenin (red, Bottom) antibodies. Nuclei were counterstained with DAPI. (Scale bar: 10 μm.)