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. 2024 Dec 23;19(12):e0316109. doi: 10.1371/journal.pone.0316109

Fig 1. ATRA-induced upregulation of NEAT1 promotes autophagy and PML/RARα degradation during granulocytic differentiation of NB4 cells.

Fig 1

(A) Schema of the two NEAT1 isoforms and the positions of qPCR primers and the shRNA targets. (B) NB4 cells were transfected with NEAT1-specific shRNA (shNEAT1) or negative control shRNA (NC). The expression levels of NEAT1 and its isoform NEAT1_2 were assessed in NB4 cells both before and after treatment with 1 μM ATRA for 48 hours by RT-qPCR. (C) The representative flow cytometry plots of NB4 cells stained with CD11b both before and after ATRA treatment. (D) The expression of granulocytic differentiation markers CD11b, CD11c and CD18 was determined in NB4 cells following treatment with 1 μM ATRA for 48 hours. (E) Protein levels of LC3B, p62 and PML/RARα were examined in NB4 cells both before and after 1 μM ATRA treatment for 48 hours. (F) The protein levels of LC3B, p62 and PML/RARα were also determined in NB4 cells treated with 1 μM ATRA treatment for 48 hours and 25 nM bafilomycin A1 for 12 hours. * indicates p<0.05.