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(A) HEK-239T were transfected with the indicated expression plasmids and collected at the specified times before fractionation, SDS-AGE and immunoblotted with an anti-GFP antibody. (B) As in (A) with cells transfected with GFP-ATXN3 polyQ for 7 days and treated with DMSO or 5 μM MG-132 for 16 hours.
