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. 2024 Dec 10;15:1491139. doi: 10.3389/fpls.2024.1491139

Figure 8.

Figure 8

Transcriptional activation activity of CnBPC4, CnBPC6A, and CnBPC7 proteins in tobacco leaves. (A) The diagram of vectors used in dual-luciferase reporter assay. The coding sequence (CDS) of CnBPC4, CnBPC6A, and CnBPC7 was cloned into the pc1300-35S vector to generate the effector. The sequence containing GA-motif - (TC)3 and (TC)11 were linked to the double-reporter vector pGreenII 0800-LUC. (B) LUC luminescence image and relative luciferase activity between GA-motif-(TC)3 and CnBPCs; and (C) between GA-motif-(TC)11 and CnBPCs. The vectors carrying the effectors were co co-transformed into Nicotiana benthamiana leaves along with the vectors carrying the reporter LUC. Three biological replicates were performed, the samples from three plants were as one replicate, and the error bars represent SE. Each value represents the means of six biological replicates. **P < 0.01 (Student’s t-test). The comparison was made between (TC)3/11-LUC + pc1300-35S and (TC)3/11-LUC+pc1300-35S-CnBPC.