Fig. 4.

Induction of early gene expression by encapsidated HPV16 genome derivatives and infectivity of encapsidated, full-length HPV31b genomic DNA. Virions were prepared as in Fig. 2 by cotransfecting HPV16 L1- and L2-expression plasmids and either the HPV16 genome deletion derivatives of Fig. 1 A (A) or the HPV31b full-length genome (B), and they were inoculated onto HaCaT cells at 100 vge per cell. After a 48-h incubation, cells were harvested, total RNA was isolated, and nested RT-PCR was performed with PCR primers to detect E1 E4 spliced mRNAs of HPV16 (A) or HPV31b (B) (see Table 1).